MALBAC® Platinum Single Cell RNA Amplification Kit specifically performs reverse transcription for mRNA in single cell or few cells, and uses patented MALBAC® Template-switching technology to add adapter containing a quantitative molecular tag to the 3' end of the cDNA, by which the 3' amplification preference and the contamination of genomic DNA and rRNA in the cDNA synthesis process are effectively avoided, so that the gene expression file is accurately retained and quantified.
Complete transcript amplification, including UTR region and UTR upstream and downstream regions, enabling full-length transcriptome sequencing
absolute quantification of gene expression by UMIs
High gene detection rate, which can analyze genes with low expression
Single cell RNA or trace RNA (10 pg of RNA in total)
2-50 ng high-quality full-length cDNA obtained from trace mRNA after reverse transcription and amplification
Rapid Procedure with 1 tube,3 steps and 5-6 hours