Technical Overview

MALBAC®(Multiple Annealing and Looping Based Amplification Cycles)multiple annealing loop amplification technology, using multiple-displacement DNA polymerase for quasi-linear whole-genome pre-amplification, and then exponential amplification by PCR technology Increase, provide sufficient experimental materials for downstream analysis.

Principle

Unlike conventiona non-linear or exponential amplification methods, MALBAC® technology uses special primers to make the ends of the amplicons complementary to form a loop, thereby largely preventing the exponential amplification bia.

Technical Advantages

Amplification success rate-the amplification success rate is higher than 96%

High uniformity-accurate copy number screening

High coverage-comprehensive detection of various single gene diseases

Low allele dropout rate-accurate detection of single-gene disease pathogenic sites

 

The picture shows the comparison of allele dropout rates of 5 single-cell amplification kits

 

The figure above shows the comparison of MALBAC® Single Cell Amplification Kit and four other common commercial single cell amplification kits in terms of allele dropout rate, amplification uniformity and coverage. The S, Q, G, YiKon, and R single cell amplification kits are based on DOP-PCR, MDA, MDA, MALBAC®, and MALBAC-like techenology. It can be seen from the above figure that the allele dropout rate (ADO) of YiKon's MALBAC® Single Cell Amplification Kit is significantly lower than the other four kits.

 
The picture shows the uniformity comparison of 5 single cell amplification kits
 
As can be seen from the figure above: The CV value of gene copy number variation (CNV) analysis issued from by YiKon's MALBAC® Single Cell Amplification Kit product is significantly lower than the other four kits, indicating its highest amplification uniformity.
 
 
The coverage comparison between MALBAC® and MDA
 
It is shown that under the same sequencing depth, the genome coverage of MALBAC® has obvious advantages over MDA andWGA4.

 

Achievement Display

19 2020-03

Validation of Multiple Annealing and Looping-based Amplification Cycle sequencing for 24-chromosome aneuploidy screening of cleavage-stage embryos

19 2020-03

Validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of blastocysts

19 2020-03

Live Births after Simultaneous Avoidance of Monogenic Diseases and Chromosome Abnormality by Next-Generation Sequencing with Linkage Analyses

19 2020-03

A New Next-generation Sequencing-based Assay for Concurrent Preimplantation Genetic Diagnosis of Charcot-Marie-Tooth Disease Type A1 and Aneuploidy Screening